Demet Akalgan
Girne American University, Cyprus
Biography
Demet Akalgan has PhD. on Pharmacognosy, Pharmacognosic Researches on Petroedmondia Syriaca (Boıss.) Tamamsch (Ph. D. Thesis) and Master of Science on Phytotherapy, Determination of Parthenolide in Tanacetum argenteum (Lam.) Willd. Subsp. Argenteum (M.Sc Thesis). 16 years of experience at Ministry of Health on regulatory affairs and regulations of medical devices, medicinal products, clinical trials and cosmetics. She has various certifications and training on European Union structure and ICH Guidelines (GMP, GDP, GCP). Ms. Akalgan is Head of Pharmacogmosy Department Girne American University, Faculty of Pharmacy and lecturing on Pharmacognosy. She has publications on breast cancer and migraine. She provides scientific support and consultation to private industry.
Abstract
The isolated and purified 12 coumarins from the roots of the plant were identified as 3'-isobutyryloxymarmesin acetate, 3′-α-angeloyloxydeltoin, smyrnioridine, deltoin, marmesin acetate, scoparone, bergapten, psoralen, colladonin, 14'-acetoxybadrakemin, marmesin and 14'-hydroxybadrakemin using their physical and spectral characteristics. Three compounds of them were isolated for the first time in this study. The extracts (hexan,CH2Cl2 etc.) were evaluated for cytotoxicity by the MTT method using MCF-7 cell line. The cytotoxic activity of the compounds obtained and the coumarin mixture were investigated and the cytotoxic activity was found to be significant. This is the first report on the chemical composition and cytotoxic activity of the roots of P. syriaca. The cytotoxic activity of the coumarin mixture was found to be significant.
The highest activity was found in the petroleum ether and dichloromethane extracts. In order to identify their cytotoxic compounds, petroleum ether and dichloromethane extracts were subjected to a series of chromatographic separations. Structures of the purified compounds were elucidated by spectroscopic methods, chemical transformations and direct comparison with the authentic standards (where available) as scoparone , psoralen , bergapten , marmesin , marmesin acetate , deltoin , smyrnioridin, 4’-acetyl-3’-isobutyroyloxymarmesin , colladonin and 14-acetoxybadrakemin .
Petroleum ether and dichloromethane extracts of Petroedmondia syriaca (Boiss.) Tamamsch. showed cytotoxic activity on MCF-7 cell lines at 25 μg/ml and higher concentrations.This is the first report on the chemical composition and cytotoxic activity of the roots of P. syriaca. The cytotoxic activity testing of the isolated compounds is currently in progress. Cytotoxic activity of colladonin has recently been reported